MCP For Claude Marketplace
Melting Temperature Calculator

Melting Temperature Calculator MCP Connector for Claude

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Calculate DNA melting temperatures, GC content, and amplicon properties for PCR optimization.

4 tools Official Updated Oct 1, 2026 Official Vinkius Partner
More Details Connect to Claude

This MCP server provides essential bioinformatics tools for PCR design. Use calculate_primer_tm to determine the thermal stability of individual primers, calculate_amplicon_properties to analyze the resulting PCR product, evaluate_mismatch_impact to predict how sequence errors affect binding, and optimize_primer_parameters to adjust salt concentrations for target melting temperatures.

pcrdnamelting-temperatureprimergenetics

4 tools expose this connector's capabilities to your AI agent.

calculate_amplicon_properties

Determines the thermal profile of the resulting PCR product (amplicon) based on two primers

calculate_primer_tm

Calculates the melting temperature and GC content for a single primer sequence

evaluate_mismatch_impact

Estimates how a specific mismatch between a primer and the template sequence will affect the melting temperature

optimize_primer_parameters

Suggests adjustments to salt concentration or sequence modifications to reach a target Tm

See how to talk to your AI agent using Melting Temperature Calculator.

What is the melting temperature for the primer sequence ATGCATGC with a salt concentration of 0.05M using the wallace method?

The melting temperature for the sequence ATGCATGC is 24.0°C with a GC content of 50%.

Calculate the properties of an amplicon with forward primer GCGCTA and reverse primer ATATAT in a template sequence GCGCTATATAT.

The amplicon has a melting temperature of 32.5°C, a length of 10bp, and a GC content of 40%.

How much should I adjust the salt concentration to reach a target Tm of 60°C for the sequence GGGCCCAAATTT?

To reach a target Tm of 60°C, the suggested salt concentration is 0.15M.

The server supports both the simplified Wallace rule and the more accurate Nearest-Neighbor model.

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